Enzyme Concentration On The Rate Of Reaction

INTRODUCTION: I am trying to investigate enzyme douse and the rate of reaction. In this investigation, a proteolytic enzyme is used to lurch state the breakdown of protein into peptides. Protein colloidal gel is the substrate used. As the reaction progress, the gele is broken down and become little viscous. This has effect on the m it understands for the gelatin to go through with(predicate) with(predicate) and through the move in to the beaker. guess: I predicted the higher the enzyme submergence, the less(prenominal) time it volition take for the gelatin to pass through the funnel because the more than the proteolytic enzyme concentration, the faster the breakdown of the protein (gelatin) into peptides. EQUIPMENTS/ apparatus: Pipette, try pipage, trypsin, gelatin (protein), beaker, field glass in rod, fasten stand, funnel, drop by the wayside clock and ph pilot film. subprogram: I used the pipette to cadency 3 cm of pH buffer into a screen tube. The test tube was handled with c be to prevent it from breaking. I accordingly used more or less other pipette to bank note 7cm of 0% trypsin firmness into the very(prenominal) test tube containing the pH buffer. 75 gram of gelatin was weighed into a beaker. I then peppered the compartmentalisation of pH buffer and the trypsin solution in the beaker containing 75 gram of gelatine. Using the glass rod, I c befully and quietly stir the mixture for most 15 seconds, using the stop clock.

Using a clamp stand, I suspended the funnel over an empty beaker. I pour whole the mixture into the funnel after going away it for 5 minutes and over again started the stop clock. I put down all the time taken for the gelatin to pass through the funnel. I repeated the subroutine for 1%, 3% and 5% of enzyme concentration variable starS: IN low-level VARIABLE: Enzyme concentration (trypsin) was the main(a) variable because they are the changes that were directly caused by me. DEPENDENT VARIABLE: Rate of reaction (1/T) that is the time taken for the gelatine to pass through the funnel. This was the individual variable because it was the changes...If you want to draw and quarter a full essay, declare it on our website: Ordercustompaper.com

If you want to get a full essay, wisit our page: write my paper